CHMP5 antibody [N1C3]
Catalogue Number: GTX106692-GTX
GTX106692 Image
GTX106692 Image
GTX106692 Image
GTX106692 Image
GTX106692 Image
GTX106692 Image
Jurkat whole cell extracts and Jurkat exosome extract (15.4 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP5 antibody [N1C3] (GTX106692) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX106692 Image
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. CHMP5 stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX106692 Image
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (GTX106692) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX106692 Image
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5(GTX106692) antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX106692 Image
Sample(30µg whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: HeLa S3 (GTX14654) E: Hep G2 (GTX27900) 12% SDS PAGE GTX106692 diluted at 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | charged multivesicular body protein 5 , C9orf83 , CGI-34 , HSPC177 , PNAS-2 , SNF7DC2 , Vps60 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM]
Additional Text
Concentration
1 mg/ml
Gene Name
CHMP5
Uniprot ID
Q9NZZ3
Purification
Affinity Purified
Gene ID
51510
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
25
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX106692 Datasheet
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