Vinculin antibody [N3C1], Internal
Catalogue Number: GTX109749-GTX
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GTX109749 Image
GTX109749 Image
GTX109749 Image
GTX109749 Image
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Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000.
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Immunohistochemical analysis of human breast cancerncer, using Vinculin(GTX109749) antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 5 % SDS-PAGE Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
GTX109749 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX109749 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary. |
| Application: | IHC-P, WB |
Description
Description: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]
Additional Text
Gene Name
VCL
Uniprot ID
P18206
Gene ID
7414
Concentration
0.11 mg/ml
Purification
Affinity Purified
Molecular Weight
124
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX109749 Datasheet
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