IL6 antibody
Catalogue Number: GTX110527-GTX
GTX110527 Image
GTX110527 Image
GTX110527 Image
GTX110527 Image
GTX110527 Image
GTX110527 Image
GTX110527 Image
IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
GTX110527 Image
Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110527 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110527 Image
Untreated (-) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110527 Image
IL6 antibody detects IL6 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. IL6 stained by IL6 antibody (GTX110527) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX110527 Image
IL6 antibody detects IL6 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL6 stained by IL6 antibody (GTX110527) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human IL6 Recombinant protein. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]
Additional Text
Gene Name
IL6
Gene ID
3569
Molecular Weight
24
Uniprot ID
P05231
Concentration
0.73 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX110527 Datasheet
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