Citrate synthase antibody [N2C3]
Catalogue Number: GTX110624-GTX
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Citrate synthetase antibody [N2C3] (GTX110624) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 µg of Citrate synthetase antibody [N2C3] (GTX110624). Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Citrate synthase antibody [N2C3] detects Citrate synthase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. Citrate synthase antibody [N2C3] (GTX110624) dilution: 1:50.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Citrate synthetase antibody detects Citrate synthetase protein by Western blot analysis. Rat tissue extracts (50 µg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) at a dilution of 1:1000.
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Citrate synthase antibody [N2C3] detects Citrate synthase protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo. Green: Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.
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Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX110624 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cs antibody (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110624 Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110624 Image
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX110624 Image
Citrate synthase antibody [N2C3] detects Citrate synthase protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | citrate synthase |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC-WM |
Description
Description: The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]
Additional Text
Gene Name
CS
Uniprot ID
O75390
Concentration
0.65 mg/ml
Purification
Affinity Purified
Gene ID
1431
Molecular Weight
52
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX110624 Datasheet
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