gamma Tubulin antibody [GTU-88]
Catalogue Number: GTX11316-GTX
GTX11316 Image
GTX11316 Image
GTX11316 Image
GTX11316 Image
ICC/IF analysis of HeLa cells using GTX11316 gamma Tubulin antibody [GTU-88] at 1:5,000. Cells were fixed and permeabilized with methanol followed by acetone.
GTX11316 Image
WB analysis of chicken fibroblasts using GTX11316 gamma Tubulin antibody [GTU-88]. Lane A : Antibody dilution 1:20,000 Lane B : Negative control (secondary antibody only)
GTX11316 Image
WB analysis of (1) HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cell lysate using GTX11316 gamma Tubulin antibody [GTU-88] at 1:10,000.
| Manufacturer: | GeneTex |
| Preservative: | 15mM Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | GTU-88 |
| Isotype: | IgG1 |
| Immunogen: | synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH. |
| Application: | ELISA, ICC, IF, WB |
Description
Description: The protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.
Additional Text
Gene ID
7283
Gene Name
TUBG1
Uniprot ID
P23258
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
51
Application Notes
WB: 1:10,000. ICC/IF: 1:5,000-1:10,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX11316 Datasheet
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