ATG5 antibody
Catalogue Number: GTX113309-GTX
GTX113309 Image
GTX113309 Image
GTX113309 Image
GTX113309 Image
GTX113309 Image
GTX113309 Image
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
GTX113309 Image
ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 µM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX113309 Image
ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ATG5 antibody (GTX113309) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX113309 Image
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Sample: HepG2 cells treated with 3µM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min. Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX113309 Image
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 12% SDS PAGE GTX113309 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, PLA |
Additional Text
Gene Name
ATG5
Uniprot ID
Q9H1Y0
Concentration
1 mg/ml
Molecular Weight
32
Gene ID
9474
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
ATG5 antibody detects autophagy related 5 (ATG5), a ~32kDa key protein that contributes to the formation of autophagic vesicles when conjugated to ATG12 through the actions of ATG7 and ATG10. It plays key roles in mitochondrial function, lymphocyte biology, and axonal maintenance, among others. Dysregulation of ATG5 is associated with several diseases, including sporadic Parkinson's disease, childhood asthma, and cancers.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX113309 Datasheet
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