GOLPH2 antibody
Catalogue Number: GTX116154-GTX
GTX116154 Image
GTX116154 Image
GTX116154 Image
GTX116154 Image
GTX116154 Image
GTX116154 Image
GTX116154 Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX116154 Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLPH2(GTX116154) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX116154 Image
GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
GTX116154 Image
HeLa whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX116154 Image
GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX116154 Image
Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human GOLPH2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]
Additional Text
Gene Name
GOLM1
Uniprot ID
Q8NBJ4
Concentration
0.16 mg/ml
Purification
Affinity Purified
Gene ID
51280
Molecular Weight
45
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116154 Datasheet
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