AKT (phospho Ser473) antibody [104A282]
Catalogue Number: GTX11901-GTX
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IHC-P analysis of human breast carcinoma tissue using GTX11901 AKT (phospho Ser473) antibody [104A282]. Dilution : 1:250 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
GTX11901 Image
IHC-P analysis of human breast carcinoma tissue using GTX11901 AKT (phospho Ser473) antibody [104A282]. Dilution : 1:250 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
GTX11901 Image
WB analysis of 3T3 cells treated with and without PDGF (50 ng/ml) using GTX11901 AKT (phospho Ser473) antibody [104A282] and AKT1 antibody. Dilution : 2 µg/ml
GTX11901 Image
WB analysis of untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate using GTX11901 AKT (phospho Ser473) antibody [104A282]. Dilution : 2 µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 104A282 |
| Isotype: | IgG1 |
| Immunogen: | This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Additional Text
Gene Name
AKT1
Concentration
1 mg/ml
Gene ID
207
Uniprot ID
P31749
Purification
Protein G purified
Modification
Phosphorylated, Ser473
Molecular Weight
56
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
IHC-P: 1:200 - 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX11901 Datasheet
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