LC3B antibody
Catalogue Number: GTX127375-GTX
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LC3B antibody detects LC3B protein in HCV-infected samples by western blot analysis. ? A. 20 µg Huh7 whole cell lysate/extract (un-infected) ? B. 20 µg Huh7 whole cell lysate/extract (HCV-infected) ? LC3B antibody (GTX127375) dilution: 1:1500 ?The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 50 µg mouse brain lysate/extract 15% SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 50 µg Rat brain lysate/extract 15% SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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LC3B antibody (GTX127375) detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody (GTX127375) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
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LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
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Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 µg of LC3B antibody (GTX127375). Western blot analysis was performed using LC3B antibody (GTX127375). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 20 µg Huh7 whole cell lysate/extract (untreated) B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr) LC3B antibody (GTX127375) dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HepG2 cells were untreated or treated with 3 µM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Wild-type (WT) and LC3B knockout (KO) 293T cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Untreated (-) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with LC3B antibody (GTX127375) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody ) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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LC3B antibody detects LC3B protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. LC3B stained by LC3B antibody (GTX127375) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Untreated (-) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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LC3B antibody detects LC3B protein at autophagosome? by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B stained by LC3B antibody (GTX127375) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
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LC3B antibody detects LC3B protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. LC3B stained by LC3B antibody (GTX127375) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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LC3B antibody detects LC3B protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. LC3B stained by LC3B antibody (GTX127375) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary. |
| Application: | FACS, ICC, IF, IHC-P, IP, EM, WB, IHC-Fr |
Description
Description: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
Additional Text
Gene Name
MAP1LC3B
Uniprot ID
Q9GZQ8
Concentration
0.34 mg/ml
Gene ID
81631
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
15
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
LC3B antibody recognizes LC3B, an autophagosomal membrane structural protein with a predicted molecular weight of 14.7 kDa. LC3B protein is a member of the highly conserved Atg8 protein family, which encompasses the LC3 and GABARAP subfamilies. The key function of LC3B involves autophagy substrate selection during autophagosome formation. Therefore, LC3B antibody is of great utility for the study of autophagosome biogenesis.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX127375 Datasheet
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