Caspase 4 antibody
Catalogue Number: GTX134552-GTX
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GTX134552 Image
GTX134552 Image
GTX134552 Image
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THP-1 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 4 (cleaved Gln81) antibody (GTX134552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX134552 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX134552) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX134552 Image
Caspase 4 antibody detects Caspase 4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Caspase 4 stained by Caspase 4 antibody (GTX134552) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX134552 Image
Caspase 4 antibody detects Caspase 4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Caspase 4 stained by Caspase 4 antibody (GTX134552) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX134552 Image
Untreated (-) and treated (+) THP-1 whole cell extract and conditioned medium (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody (GTX134552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | caspase 4 , ICE(rel)II , ICEREL-II , ICH-2 , Mih1 , Mih1/TX , TX |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caspase 4. The exact sequence is proprietary. |
| Application: | IHC-P, WB |
Description
Description: This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
CASP4
Uniprot ID
P49662
Gene ID
837
Concentration
1.12 mg/mL
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
43
Application Notes
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX134552 Datasheet
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