Respiratory Syncytial virus type A Nucleoprotein, DDDDK tag

Catalogue Number: GTX136751-PRO-GTX

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GTX136751-pro Respiratory syncytial virus type A Nucloprotein, DDDDK tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

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Respiratory Syncytial virus type A Nucleoprotein, DDDDK tag (1 µg, GTX136751-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 µg/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 µg/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.

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Sandwich ELISA detection of recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) (5 µg/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (1 µg/mL) Please notice that GTX636649 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636709. Please contact us for custom HRP-conjugated antibody.

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Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 µg/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (1 µg/mL) Please notice that GTX636648 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636650. Please contact us for custom HRP-conjugated antibody.

GTX136751-PRO Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 µg/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (1 µg/mL) Please notice that GTX636648 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636649. Please contact us for custom HRP-conjugated antibody.

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Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX136751-PRO Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (GTX636647) (5 µg/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) (1 µg/mL) Please notice that GTX636705 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636647. Please contact us for custom HRP-conjugated antibody.

GTX136751-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 µg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) 3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

GTX136751-PRO Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 µg/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 µg/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.

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Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair. Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248]) Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246]) Samples (25 ng) : 1. RSV type A Nucleoprotein (GTX136751-pro) 2. RSV type B Nucleoprotein (GTX136931-pro) 3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro) 4. FluA (H1N1) Nucleoprotein (GTX135904-pro) 5. FluA (H3N2) Nucleoprotein (GTX135903-pro) 6. FluB Nucleoprotein (GTX136277-pro) 7. Lysis buffer

GTX136751-PRO Image

Sandwich ELISA detection of recombinant Respiratory Syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) (5 µg/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (1 µg/mL) Please notice that GTX636649 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636709. Please contact us for custom HRP-conjugated antibody.

GTX136751-PRO Image

Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 µg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

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Manufacturer:	GeneTex
Preservative:	no preservative\|No Preservative
Physical state:	Liquid
Type:	Virus Proteins
Host Cell:	HEK293 cells
Shipping Condition:	Dry Ice
Unit(s):	100 ug
Application:	ELISA, sELISA, WB, LF

Overview
Datasheets

Additional Text

Concentration

0.2 mg/ml

Protein Tag

FLAG™ Tag

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

Recommended antibody pairs for Lateral Flow: Capture : GTX638184, Detection : GTX638186, or Capture : GTX636650, Detection : GTX636648 Recommended antibody pairs for sandwich ELISA: Capture : GTX636711 / GTX636649, Detection : GTX640511, or Capture : GTX636650 / GTX636649, Detection : GTX636648, or Capture : GTX636709, Detection : GTX636649, or Capture : GTX636647, Detection : GTX636705 Please notice that the detection antibody needs to be conjugated to HRP when paired with the capture antibody. Please contact us for custom HRP-conjugated antibody.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70C or below. Do not vortex.

GTX136751-PRO Datasheet