Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag
Catalogue Number: GTX136931-PRO-GTX
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GTX136931-PRO Image
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GTX136931-PRO Image
GTX136931-PRO Image
GTX136931-PRO Image
GTX136931-pro Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
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Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (1 µg, GTX136931-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair. Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248]) Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246]) Samples (25 ng) : 1. RSV type A Nucleoprotein (GTX136751-pro) 2. RSV type B Nucleoprotein (GTX136931-pro) 3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro) 4. FluA (H1N1) Nucleoprotein (GTX135904-pro) 5. FluA (H3N2) Nucleoprotein (GTX135903-pro) 6. FluB Nucleoprotein (GTX136277-pro) 7. Lysis buffer
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Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
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Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (GTX636647) (5 µg/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) (1 µg/mL) Please notice that GTX636705 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636647. Please contact us for custom HRP-conjugated antibody.
GTX136931-PRO Image
Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 µg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) 3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)
GTX136931-PRO Image
Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 µg/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 µg/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Virus Proteins |
| Host Cell: | HEK293 cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ug |
| Application: | ELISA, sELISA, WB, LF |
Additional Text
Concentration
0.4 mg/ml
Protein Tag
FLAG™ Tag
Application Notes
Recommended antibody pairs for Lateral Flow: Capture : GTX638184 / GTX636649, Detection : GTX638186
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70C or below. Do not vortex.
GTX136931-PRO Datasheet
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