Catalogue Number: GTX16373-GTX
Manufacturer: | GeneTex |
Type: | Protein Extraction Kit |
Shipping Condition: | Dry Ice |
Unit(s): | 20 tests, 5 tests |
Application: | ELISA, IP, WB |
Description: Trident Membrane protein extraction kit is for rapid extraction of native total membrane proteins from cultured mammalian cells or tissues. Simple and user friendly Wide range of starting cells (1 - 50 millions / sample) Detergent and EDTA free No need for Dounce homogenizer or tissue blender The procedure can be completed in less than 45 min. High yield
1. Read the entire protocol carefully. 2. Before experiment, thaw buffer A or/and buffer B completely, invert the bottles a few times and keep them on ice. Place the filter cartridges in collection tubs, and pre-chill on ice. Pre-chill PBS on ice. 3. For IP, dissolve the extracted plasma membrane in PBS containing 0.5% Triton-X 100. Then do IP assay.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Spin column-based protein extraction and cell fractionation technologies were developed by Invent Biotechnologies, Inc.