EasyBlot anti-Rabbit IgG Kit (Optimized for Prot A/G)

Catalogue Number: GTX225856-01-GTX

Manufacturer:	GeneTex
Preservative:	0.005% Thimerosal
Type:	IHC kits
Shipping Condition:	Dry Ice
Unit(s):	250 ul, 50 ul
Application:	WB

Overview
Datasheets

Additional Text

Purification

Protein G purified

Concentration

0.318 mg/ml

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

Suggested starting dilutions for WB is 1:1000. Use 2-5 µg of the IP antibody; do not exceed 5 µg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Short Description

EasyBlot anti-Rabbit IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an E

GTX225856-01 Datasheet

GTX225856-01 Reference

Price

£307.00

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