Catalogue Number: GTX23331-GTX
Manufacturer: | GeneTex |
Preservative: | 0.05% Sodium azide |
Physical state: | Liquid |
Type: | Polyclonal Primary Antibody - Unconjugated |
Alias: | proteasome (prosome, macropain) subunit, beta type 6 , Lmp19 , Mpnd |
Shipping Condition: | Blue Ice |
Unit(s): | 100 ug |
Host name: | Rabbit |
Clone: | |
Isotype: | IgG |
Immunogen: | Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58) |
Application: | ICC, IF, WB |
Description: Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).
1 mg/ml
PSMB6
Affinity Purified
19175
Q60692
Polyclonal
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
25
WB: 1 µg/ml. ICC/IF: 1:10-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.