Fibrillarin antibody [38F3]
Catalogue Number: GTX24566-GTX
GTX24566 Image
GTX24566 Image
GTX24566 Image
GTX24566 Image
GTX24566 Image
Blot of yeast protein extract stained with the GTX24566 anti-Gibrillarin Antibody, stained a single band at ~34kDa.
GTX24566 Image
Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000
GTX24566 Image
High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixat
GTX24566 Image
Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue).
| Manufacturer: | GeneTex |
| Preservative: | 10mM Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | fibrillarin , FIB , FLRN , Nop1 , RNU3IP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 250 ul |
| Host name: | Mouse |
| Clone: | 38F3 |
| Isotype: | IgG1 |
| Immunogen: | Yeast nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae). |
| Application: | ICC, IF, IHC-P, WB, IHC |
Description
Description: This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
FBL
Gene ID
2091
Purification
Unpurified
Uniprot ID
P22087
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
34
Application Notes
IF (ICC): 1/1000 - 1/5000 (yeast cells). For IF of mammalian cells, 1/500. WB: 1/2000 (cell lysates) - 1/10000 (nuclear fractions)(ECL). Detects a band of approximately 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000. To detect mammalian fibrillarin on western blots by ECL, 1/500. Optimal dilutions/concentrations should be determined by the end user.
GTX24566 Datasheet
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