FAK (phospho Tyr861) antibody
Catalogue Number: GTX24804-GTX
GTX24804 Image
GTX24804 Image
GTX24804 Image
WB (peptide competition) analysis of 3T3-L1 cells stimulated with 50 ng/ml LIF for 15 minutes (Lane 2-4) using GTX24804 FAK (phospho Tyr861) antibody prior incubated with the phosphopeptide immunogen (Lane 1), or genertic phosphopeptide (Lane 2), the non-phosphopeptide corresponding to generic phosphoserine-containing peptide (Lane 3), or no peptide (Lane 4).. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
GTX24804 Image
ICC/IF analysis of A549 cells using GTX24804 FAK (phospho Tyr861) antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton X-100 for 10 minutes Dilution : 1 µg/ml in 1% BSA incubated for 3 hours at room temperature
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC |
Description
Description: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
Additional Text
Gene Name
PTK2
Gene ID
5747
Uniprot ID
Q05397
Purification
Affinity Purified
Modification
Phosphorylated, Tyr861
Molecular Weight
119
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000. ICC/IF: 1 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX24804 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations