SNAP25 antibody
Catalogue Number: GTX25666-GTX
GTX25666 Image
GTX25666 Image
GTX25666 Image
WB analysis of tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2), Rat Brain (Lane 3) and membrane enriched extract (30 µg lysate) of PC-12 (Lane 4) using GTX25666 SNAP25 antibody. Dilution : 1 µg/ml
GTX25666 Image
ICC/IF analysis of cultured rat hippocampal cells using GTX25666 SNAP25 antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | synaptosome associated protein 25a , Snap , fj42b02 , fj45g04 , fj48c02 , snap25 , snap25.1 , wu:fj42b02 , wu:fj45g04 , wu:fj48c02 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic Peptide:(C)- A(195) N Q R A T K M L G S G(206) |
| Application: | FACS, ICC, IF, WB, IHC, NT, Inh |
Description
Description: Synaptosomal associated protein of 25 kDa (SNAP-25) is a soluble protein of 25 kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. This protein was originally discovered to be enriched in subsets of neurons in the brain and displays a presynaptic pattern of expression. The membrane proteins SNAP-25, synaptobrevin, and syntaxin form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. Functional interactions have been demonstrated to exist between SNAP-25, syntaxin, and the synaptic protein interaction site on voltage-sensitive L- and N-type calcium channels. SNAP-25 has been shown to be required for synaptic vesicle fusion with the plasma membrane during release of nerve growth factor (NGF). Regulated exocytosis of cortical granule secretion in fertilized eggs and membrane fusion events in neurons and endocrine cells is mediated by SNAP-25 in a calcium-dependent mechanism. SNAP-25 protein levels have been shown to be elevated in prolactinoma and growth hormone (GH)/prolactin (PRL) tumor cells while reduced SNAP-25 protein expression has been observed between schizophrenic and normal hippocampal cells. Altered patterns of SNAP-25 expression have also been observed in the inferior temporal cortex and prefrontal association cortex between normal brains and brains from individuals affected with schizophrenia. SNAP-25 accumulation due to cytoskeletal dysfunction is also observed in the swollen axons of the white matter of individuals with severe Alzheimer's dementia.
Additional Text
Concentration
1 mg/ml
Purification
Affinity Purified
Gene ID
30712
Uniprot ID
Q5TZ66
Gene Name
snap25a
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
23
Application Notes
WB: 0.5-2 µg/ml. ICC/IF: 1:100. FACS: 3-5 µg x 10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX25666 Datasheet
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