Pepsin (ready to use)

Catalogue Number: GTX28194-GTX

Manufacturer:	GeneTex
Physical state:	Liquid
Type:	Proteases
Shipping Condition:	Blue Ice
Unit(s):	50 ml
Application:	IHC-P, IHC

Overview
Datasheets

Description

Description: Most fixatives immobilize the tissue antigens by cross linkages. Sometimes, extensive cross linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to a certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, keratins, carcinoembryonic antigen, factor VIII related antigen, testosterone, and to a lesser extent S100 protein.

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

This antigen retrieval solution is provided in a ready-to-use format requiring no further preparation. 1.) Apply this solution to tissue sections following their initial hydration step. Incubate for 10 min at 37C or 45C (this incubation time may vary depending on the degree of fixation of the tissue sections. 2.) Wash tissue sections well with IHC wash buffer (GTX28195), or PBS or TBS if immunostaining by hand.

Short Description

Antigen retrieval solution-enzymatic (pepsin)

GTX28194 Datasheet

GTX28194 Reference

Price

£345.00

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