AEC
Catalogue Number: GTX30938-GTX
| Manufacturer: | GeneTex |
| Physical state: | Liquid |
| Type: | Enzyme Substrates |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 kit |
| Application: | IHC |
Description
Description: Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives bright red color and is very compatible with hematoxylin nuclear counterstain. This chromogen is soluble in alcohol and organic solvents therefore cannot be mounted with organic mounting medium. We recommend our aqueous mounting medium ImmunoHistoMount (IHM) for mounting of this chromogen.
Additional Text
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. Add one drop of reagent C chromogen, mix well. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country's regulations. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/ deionized water. Add few drops of ready to use AEC reagent, incubate at RT or 37C for 5-10 minutes. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. Add counterstain compatible with AEC (we recommend hematoxylin counterstain for 30-60 seconds). Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
Short Description
AEC
GTX30938 Datasheet
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