Grp78 antibody
Catalogue Number: GTX48633-GTX
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FACS (Intracellular staining) analysis of HeLa cells using GTX48633 Grp78 antibody. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA Permibilization : 0.1% saponin
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FACS (Intracellular staining) analysis of Jurkat cells using GTX48633 Grp78 antibody. Blue : Primary antibody Orange : isotype control Dilution : 2 µg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
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WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.
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WB analysis of HeLa cell lysate using GTX48633 Grp78 antibody.
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WB analysis of HeLa, A431, NIH-3T3, PC12 cell lysate using GTX48633 Grp78 antibody. Dilution : 1 µg/ml
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ICC/IF analysis of HeLa cells using GTX48633 Grp78 antibody. Green : primary antibody Red : Tubulin Blue : DAPI Dilution : 5 µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021] |
| Application: | FACS, ICC, IF, WB |
Description
Description: When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Additional Text
Gene Name
HSPA5
Concentration
1 mg/ml
Gene ID
3309
Uniprot ID
P11021
Purification
Affinity Purified
Molecular Weight
72
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.5 µg/ml. ICC/IF: 1:50. FACS: 1:150. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX48633 Datasheet
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