Cas9 antibody [7A9]
Catalogue Number: GTX53807-GTX
GTX53807 Image
GTX53807 Image
GTX53807 Image
ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 µg/ml (green) and DAPI (blue).
GTX53807 Image
IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 25 ug |
| Host name: | Mouse |
| Clone: | 7A9 |
| Isotype: | IgG1 |
| Immunogen: | N-Terminal domain of Cas9. |
| Application: | ICC, IF, IP, WB |
Additional Text
Purification
Protein G purified
Gene ID
901176
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
cas9
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Cas9 in 1 µg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
GTX53807 Datasheet
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