SDHA antibody [GT20710]
Catalogue Number: GTX632636-GTX
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.
GTX632636 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.
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SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
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SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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HepG2 and mitochondria extracts (30 µg) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX632636 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX632636 Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX632636 Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX632636 Image
SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT20710 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]
Additional Text
Gene Name
SDHA
Uniprot ID
P31040
Concentration
1 mg/ml
Gene ID
6389
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
73
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX632636 Datasheet
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