LAMP1 antibody [GT25212]
Catalogue Number: GTX634336-GTX
GTX634336 Image
GTX634336 Image
GTX634336 Image
GTX634336 Image
GTX634336 Image
GTX634336 Image
GTX634336 Image
LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:2000. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX634336 Image
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX634336 Image
Wild-type (WT) and LAMP1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX634336 Image
LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX634336 Image
LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX634336 Image
HeLa whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract) *The competitor is not affiliated with GeneTex and does not endorse this product.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | lysosomal associated membrane protein 1 , CD107a , LAMPA , LGP120 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT25212 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human LAMP1. The exact sequence is proprietary. |
| Application: | ICC, IF, WB, IHC-Fr |
Description
Description: The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]
Additional Text
Gene Name
LAMP1
Concentration
1 mg/ml
Gene ID
3916
Uniprot ID
P11279
Molecular Weight
45
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
LAMP1 antibody detects lysosome-associated membrane protein 1 (LAMP-1, or CD107a), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP1 and LAMP2 are major protein components of lysosomal membranes, though both proteins appear to have a more dynamic distribution that includes endosomes, autolysosomes, and multivesicular bodies. Nevertheless, LAMP1 is often used as an indicator of lysosomes in cells.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX634336 Datasheet
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