ASFV p54 antibody [GT853]
Catalogue Number: GTX635690-GTX
GTX635690 Image
GTX635690 Image
GTX635690 Image
GTX635690 Image
GTX635690 Image
GTX635690 Image
GTX635690 Image
GTX635690 Image
GTX635690 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT853] (GTX635690) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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ASFV p54 antibody [GT853] detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody [GT853] (GTX635690) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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ASFV p54 antibody [GT853] detects ASFV p54 protein at cytoplasm by immunohistochemical analysis. Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block. Green: ASFV p54 stained by ASFV p54 antibody [GT853] (GTX635690) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [GT853] (GTX635690) (5 µg/mL) Detection: HRP-conjugated ASFV p54 antibody [GT1075] (GTX635691) (1 µg/mL) Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.
GTX635690 Image
Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [GT853] (GTX635690) (5 µg/mL) Detection: ASFV p54 antibody (GTX135175) (1 µg/mL).
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Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro, 0.2 µg/ml) by lateral flow assay. Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 µg/ml). Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 µg/ml).
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Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (GTX635690 [GT853]) Detection: ASFV p54 antibody (GTX636703 [HL1289]) Samples (40 ng) : 1. ASFV p54 (ECD) protein (GTX137950-pro) 2. Lysis buffer
GTX635690 Image
Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (GTX635690 [GT853]) Detection: ASFV p54 antibody (GTX636562 [HL1218]) Samples (40 ng) : 1. ASFV p54 (ECD) protein (GTX137950-pro) 2. Lysis buffer
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 20 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | African swine fever virus p54 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT853 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of ASFV p54 (ASFV (strain: BA71V)). The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, sELISA, WB, LF |
Additional Text
Concentration
1 mg/ml
Molecular Weight
20
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
ASFV p54 antibody detects envelope protein p54 of African swine fever virus. Protein p54, encoded by the E183L gene, is a 20 kDa type I transmembrane protein that localizes to envelope precursors derived from the host ER. p54 is involved in intracellular microtubule-dependent transport of viral capsid toward viral factories, and is detected on membrane-like structures within viral factories and mature extracellular virions. This is a valuable mouse monoclonal antibody for research into this very contagious hemorrhagic disease of pigs.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX635690 Datasheet
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