Influenza B virus Nucleoprotein antibody [HL1069]

Catalogue Number: GTX636100-GTX

GTX636100 Image

Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

GTX636100 Image

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636100 Image

Influenza B viral lysates (1 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636100 Image

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 µg/mL) Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 µg/mL) Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 µg/mL) Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 µg/mL) Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 µg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX636100 Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 µg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 µg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX636100 Image

Influenza B virus Nucleoprotein antibody (GTX636100) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:2000.

GTX636100 Image

Immunofluorescent analysis of influenza virus infected cells using Influenza B virus Nucleoprotein antibody (GTX636100). Sample: Influenza A and B Virus infected cells slide. Green: Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:100.

GTX636100 Image

Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

GTX636100 Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 µg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 µg/mL. Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 µg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 µg/mL. Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 8.72 pM

GTX636100 Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 µg/mL). Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 µg/mL). Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 µg/mL) Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below. Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 µg/mL) Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 µg/mL) Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636100 Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza B virus nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) (50 ng), and probing with the specified influenza B virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Previous Next

Manufacturer:	GeneTex
Preservative:	no preservative\|No Preservative
Physical state:	Liquid
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 25 ul
Host name:	Rabbit
Clone:	HL1069
Isotype:	IgG
Immunogen:	Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.
Application:	ELISA, ICC, IF, sELISA, WB, LF

Overview
Datasheets

Additional Text

Concentration

1 mg/ml

Purification

Protein A purified

Antibody Clonality

Recombinant Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX636100 Datasheet