Influenza A virus Nucleoprotein antibody [HL1103]

Catalogue Number: GTX636318-GTX

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Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

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Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)

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Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Influenza A virus Nucleoprotein antibody [HL1089] detects Influenza A virus Nucleoprotein protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded flu A_NP (Kansas/2017/H3N2) transfected 293T cell FFPE Cell Pellet Block. Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [HL1089] (GTX636318) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (GTX135903-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 139.15 pM

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Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987)) using antibodies as below. Capture : Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 µg/mL) Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 µg/mL). Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

GTX636318 Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture: Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (5 µg/mL) Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 µg/mL). Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636199. Please contact us for custom HRP-conjugated antibody.

GTX636318 Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture: Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (5 µg/mL) Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 µg/mL). Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636247. Please contact us for custom HRP-conjugated antibody.

GTX636318 Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture: Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 µg/mL) Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 µg/mL). Please notice that GTX636199 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

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Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/Singapore/63/04 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 µg/mL) Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 µg/mL) Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

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Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 µg/mL) Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 µg/mL) Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

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Influenza A virus Nucleoprotein antibody (GTX636318) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636318) diluted at 1:2000.

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Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein protein (A/California/2009 (H1N1)), DDDDK Tag (GTX135904-pro) using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1098] (GTX636282) (5 µg/mL) Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (1 µg/mL) Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636282. Please contact us for custom HRP-conjugated antibody.

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Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636318). Sample: Influenza A and B Virus infected cells slide. Green: Influenza A virus Nucleoprotein antibody (GTX636318) diluted at 1:100.

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Indirect ELISA analysis was performed by coating a plate with viral lysates (1 µg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 µg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

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Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX636318 Image

Influenza A viral lysate (1 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636318 Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 µg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

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Manufacturer:	GeneTex
Preservative:	no preservative\|No Preservative
Physical state:	Liquid
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 25 ul
Host name:	Rabbit
Clone:	HL1103
Isotype:	IgG
Immunogen:	Recombinant protein encompassing a sequence within the center region of Influenza A virus Nucleoprotein (A/Kansas/14/2017(H3N2)). The exact sequence is proprietary.
Application:	ELISA, ICC, IF, sELISA, WB, IHC, LF

Overview
Datasheets

Additional Text

Concentration

1 mg/ml

Purification

Protein A purified

Antibody Clonality

Recombinant Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX636318 Datasheet