ASFV p54 antibody [HL1219]
Catalogue Number: GTX636563-GTX
GTX636563 Image
GTX636563 Image
GTX636563 Image
GTX636563 Image
GTX636563 Image
GTX636563 Image
GTX636563 Image
Indirect ELISA analysis performed by coating plate with recombinant recombinant HEK293 expressed, full-length ASFV p54 (40-0.63 nM). Coated protein was probed with ASFV p54 antibody [HL1219] (GTX636563) (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
GTX636563 Image
Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (GTX636563 [HL1219]) Detection: ASFV p54 antibody (GTX636703 [HL1289]) Samples (20 ng) : 1. ASFV p54 (ECD) protein (GTX137950-pro) 2. Lysis buffer
GTX636563 Image
Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (GTX636563 [HL1219]) Detection: ASFV p54 antibody (GTX636562 [HL1218]) Samples (20 ng) : 1. ASFV p54 (ECD) protein (GTX137950-pro) 2. Lysis buffer
GTX636563 Image
Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1218] (GTX636562) (5 µg/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1219] (GTX636563) (0.1 µg/mL) Please notice that GTX636563 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636562. Please contact us for custom HRP-conjugated antibody.
GTX636563 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1219] (GTX636563) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636563 Image
ASFV p54 antibody [HL1219] detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody [HL1219] (GTX636563) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10µm.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1219 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of ASFV p54. (African swine fever virus (strain: BA71V)) |
| Application: | ELISA, ICC, IF, sELISA, WB, LF |
Additional Text
Concentration
1 mg/ml
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636563 Datasheet
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