Catalogue Number: GTX636650-GTX
Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 µg/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 µg/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 µg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) 3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)
Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 µg/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 µg/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] detects Respiratory Syncytial virus Nucleoprotein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Respiratory Syncytial virus Nucleoprotein stained by Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair. Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248]) Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246]) Samples (25 ng) : 1. RSV type A Nucleoprotein (GTX136751-pro) 2. RSV type B Nucleoprotein (GTX136931-pro) 3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro) 4. FluA (H1N1) Nucleoprotein (GTX135904-pro) 5. FluA (H3N2) Nucleoprotein (GTX135903-pro) 6. FluB Nucleoprotein (GTX136277-pro) 7. Lysis buffer
Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 µg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.
Indirect ELISA analysis performed by coating plate with recombinant E.coli expressed, C-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) 3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)
Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus nucleoprotein using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 µg/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP (1 µg/mL) Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648) (5 µg/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 µg/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 µg/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 µg/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 µg/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.Please contact us for custom HRP-conjugated antibody.
Manufacturer: | GeneTex |
Preservative: | no preservative|No Preservative |
Physical state: | Liquid |
Type: | Recombinant Monoclonal |
Shipping Condition: | Blue Ice |
Unit(s): | 100 ul, 25 ul |
Host name: | Rabbit |
Clone: | HL1248 |
Isotype: | IgG |
Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of Respiratory Syncytial virus Nucleoprotein. (strain A2) |
Application: | ELISA, ICC, IF, IP, sELISA, WB, LF |
1 mg/ml
Protein A purified
Recombinant Monoclonal
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
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