Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502]
Catalogue Number: GTX636976-GTX
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GTX636976 Image
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GTX636976 Image
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976). Sample: Multiple virus infected cells slide. Green: Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).
GTX636976 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636976 Image
Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX636976 Image
Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and VEEV nsp3 (Venezuelan equine encephalitis virus) - transfected 293T cell pellet. Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1502 |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment of Venezuelan Equine Encephalitis Virus nsP3 (Strain P676). |
| Application: | ICC, IF, WB, IHC |
Additional Text
Concentration
1 mg/ml
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX636976 Datasheet
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