Human Papillomavirus type 16 E7 antibody [HL1821]
Catalogue Number: GTX637546-GTX
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Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair. Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647]) Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821]) Samples (10 ng) : 1. HPV16 E7 protein (GTX133411-pro) 2. HPV18 E7 protein (GTX133412-pro) 3. Lysis buffer
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Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 µg/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 µg/mL) Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.
GTX637546 Image
Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 µg/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 µg/mL) Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | HL1821 |
| Isotype: | IgG |
| Immunogen: | Full length Human Papillomavirus type 16 E7 recombinant protein |
| Application: | ELISA, sELISA, WB, LF |
Additional Text
Concentration
2 mg/ml
Gene ID
1489079
Gene Name
E7
Uniprot ID
P03129
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX637546 Datasheet
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