TSG101 antibody [4A10]
Catalogue Number: GTX70255-GTX
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GTX70255 Image
GTX70255 Image
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GTX70255 Image
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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TSG101 antibody [4A10] detects TSG101 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 10% SDS-PAGE TSG101 antibody [4A10] (GTX70255) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX70255 Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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TSG101 antibody [4A10] (GTX70255) detects TSG101 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: TSG101 antibody [4A10] (GTX70255) dilution: 1:25. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
GTX70255 Image
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 antibody [4A10] (GTX70255) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX70255 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (GTX70255) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX70255 Image
ELISA detection of TSG101 using GTX118736 for capture at a concentration of 5 µg/mL and GTX70255 for detection at a concentration of 1.5 µg/mL.
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | tumor susceptibility 101 , TSG10 , VPS23 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 4A10 |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 167-374 of TSG101 protein expressed in E. coli. |
| Application: | ELISA, FACS, ICC, IF, IHC-P, IP, EM, WB, IHC, IHC-Flt |
Description
Description: The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
TSG101
Uniprot ID
Q99816
Gene ID
7251
Concentration
1.8 mg/mL
Purification
Affinity Purified
Molecular Weight
44
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
TSG101 antibody [4A10] is a well-validated and highly cited mouse monoclonal antibody that detects TSG101, a protein of 390 amino acids and a predicted molecular weight of 44 kDa that is a core constituent of the ESCRT-I complex. Reports have described it having both tumor suppressor and tumor enhancer activities. TSG101 is involved in exosome secretion and is commonly used as an exosome marker.
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:500-1:1000. IHC-P: 1:100-1:1000. FACS: 1:25-1:200. EM: . IHC: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX70255 Datasheet
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