BCIP/NBT chromogen/substrate

Catalogue Number: GTX73326-GTX

Manufacturer:	GeneTex
Type:	Immunodetection Kits and Reagents
Shipping Condition:	Blue Ice
Unit(s):	15 ml
Application:	IHC

Overview
Datasheets

Description

Description: Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization and DNA sequencing. Detection of this enzyme requires the use of an insoluble colored substrate. GTX73326 is a new stable, BCIP/NBT solution in ready to use form. It produces a blue colored end product at the positive sites which gives a good contrast with red nuclear fast counter stain. Specimens stained in BCIP/NBT can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

1. Once tissue sections have been incubated with alkaline phosphatase, wash them with buffer thoroughly. 2. Wipe the glass to remove excess of buffer and add enough drops of the BCIP/NBT solution to cover the tissue sections. 3. Incubate for 10-30 minutes at room temperature. For the best results, look under the microscope for the signal development. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in the wash buffer.

Short Description

Alkaline Phosphatase Substrate-Chromogen (BCIP/NBT)

GTX73326 Datasheet

Price

£197.00

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