Actin antibody [mAbGEa]
Catalogue Number: GTX80809-GTX
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IHC-P analysis of human colon carcinoma tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
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IHC-P analysis of human skeletal muscle tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
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IHC-P analysis of human tonsil tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
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ICC/IF analysis of 3T3 cells using GTX80809 Actin antibody [mAbGEa]. Green : Primary antibody Blue : cell nucleus Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature
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ICC/IF analysis of A375 Cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4C
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ICC/IF analysis of HeLa cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4C
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | ChIP Validated Antibodies |
| Alias: | actin alpha cardiac muscle 1 , ACTC , ASD5 , CMD1R , CMH11 , LVNC4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | mAbGEa |
| Isotype: | IgG1 |
| Immunogen: | Purified Arabidopsis actin protein. |
| Application: | ELISA, ICC, IF, IHC-P, IP, ChIP, WB, IHC |
Description
Description: Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
ACTC1
Molecular Weight
42
Uniprot ID
P68032
Gene ID
70
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1,000. ICC/IF: 1:10-1:100. IHC-P: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX80809 Datasheet
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