Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Low Endotoxin
Catalogue Number: ICH1091-ICB
| Manufacturer: | IchorBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Programmed cell death protein 1, Pdcd1, CD279 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | Rat |
| Clone: | 29F.1A12 |
| Isotype: | IgG2a, kappa |
| Immunogen: | PD-1 cDNA followed by PD-1-Ig fusion protein |
| Application: | FC, WB, IHC-Fr, Blk |
Description
Description: Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
Additional Text
Gene Name
PDCD1
Purification
Protein A/G Purified
Gene ID
18566
Uniprot ID
Q02242
Caution
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Concentration
1-5 mg/ml
Antibody Clonality
Monoclonal
Application Notes
Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.
Storage Note
anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
ICH1091 Datasheet
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