RNase Inhibitor, Murine
Catalogue Number: K1046-APE
| Manufacturer: | Apexbio |
| Type: | Ribonuclease Inhibitors |
| Shipping Condition: | Dry Ice |
| Unit(s): | 15000 units, 3000 units |
Description
Description: RNase Inhibitor, Murine is a 50 kD recombinant protein isolated from E. coli containing the ribonuclease inhibitor gene originally from mouse. Our product RNase inhibitor has specificity for RNases A, B and C, but couldn't inhibit RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. It can bind several kinds of RNase non-covalently in a 1:1 ratio with high affinity. Especially, according to previous researches, no inhibition for enzyme activity is observed when RNase Inhibitor is used within AMV or M-MuLV reverse transcriptases, taq DNA polymerase or SP6/T7/T3 RNA polymerases, means it can be taken in various experiments to prevent RNA degradation.Compared to RNase from human, RNase inhibitor from mouse lacks the pair of cysteines which is very sensitive to oxidation and lead to inactivation of the inhibitor. As a consequence, RNase Inhibitor, Murine significantly improves its resistance to oxidation compared to RNase inhibitors from human/porcine. It is stable at low DTT concentrations (less than 1 mM), and this character makes it preferred for reactions where low concentration DTT is necessary to the reaction (eg. Real-time RT-PCR). Our product can be used in many kinds of reactions to prevent RNA degradation, for example, RT-PCR, cDNA synthesis, In vitro transcription, enzymatic RNA labeling reaction or any other applications.
Additional Text
Short Description
RNase Inhibitor, Murine specifically inhibits RNases A, B and C to protect RNA from degradation.
Storage Note
Store the components at -20°C.
K1046 CofA
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