HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A))
Catalogue Number: K1064-APE
| Manufacturer: | Apexbio |
| Type: | mRNA Synthesis kit |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 reactions |
Description
Description: HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A)) is designed for quick production of ARCA capped, 5mCTP and ψUTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) / ψUTP (Catalog No. B7972) can reduce host cell immune response. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.The kit contains sufficient reagents to carry out 25 reactions, 20 µL each time. Up to 25-180 µg of RNA can be generated with 1 µg of control template per standard reaction.
Additional Text
Short Description
Used for the in vitro synthesis of mRNA (ARCA capped, 5mCTP and ψUTP modified, poly(A) tailed)
Storage Note
Store all kit components at -20°C.
K1064 Protocol
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