HyperScribe™ All in One mRNA Synthesis Kit Plus 2 (ARCA, 5-moUTP, T7, poly(A))

Catalogue Number: K1065-APE

Manufacturer:	Apexbio
Type:	mRNA Synthesis kit
Shipping Condition:	Dry Ice
Unit(s):	25 reactions

Overview
Datasheets

Description

Description: HyperScribe™ All in One mRNA Synthesis Kit Plus 2 (ARCA, 5-moUTP, T7, poly(A)) is designed for quick production of ARCA capped, 5-moUTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5-moUTP (Catalog No. B8061) can suppress RNA-mediated innate immune activation. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.The kit contains sufficient reagents to carry out 25 reactions, 20 µL each time. Up to 25-180 µg of RNA can be generated with 1 µg of control template per standard reaction.

Additional Text

Short Description

Used for the in vitro synthesis of mRNA (ARCA capped, 5-moUTP modified, poly(A) tailed)

Storage Note

Store all kit components at -20°C.

K1065 Protocol

Price

£440.00

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