HyperScribe™ All in One mRNA Synthesis Kit II Plus 1 (EZ Cap Reagent AG (3' OMe), 5mCTP, ψUTP, T7, poly(A))

Catalogue Number: K1067-APE

Manufacturer:	Apexbio
Type:	mRNA Synthesis kit
Shipping Condition:	Dry Ice
Unit(s):	25 reactions

Overview
Datasheets

Description

Description: HyperScribe™ All in One mRNA Synthesis Kit II Plus 1 (EZ Cap Reagent AG (3' OMe), 5mCTP, ψUTP, T7, poly(A)) is designed to synthesize EZ Cap Reagent AG (3' OMe) capped, 5mCTP and ψUTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of capping reagent (EZ Cap Reagent AG (3' OMe), Catalog No. B8178) using T7 RNA Polymerase. The capping of EZ Cap Reagent AG (3' OMe) results in a Cap 1 structure and ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) / ψUTP (Catalog No. B7972) can reduce host cell immune response. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.The kit contains sufficient reagents to carry out 25 reactions, 20 µL each time. Up to 25-180 µg of RNA can be generated with 1 µg of control template per standard reaction.

Additional Text

Short Description

Used for the in vitro synthesis of mRNA (EZ Cap Reagent AG (3' OMe) capped, 5mCTP and ψUTP modified, poly(A) tailed)

Storage Note

Store all kit components at -20°C.

K1067 Protocol

Price

£580.00

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