HyperScribe™ All in One mRNA Synthesis Kit II Plus 2 (EZ Cap Reagent AG (3' OMe), 5-moUTP, T7, poly(A))
Catalogue Number: K1068-APE
| Manufacturer: | Apexbio |
| Type: | mRNA Synthesis kit |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 reactions |
Description
Description: HyperScribe™ All in One mRNA Synthesis Kit II Plus 2 (EZ Cap Reagent AG (3' OMe), 5-moUTP, T7, poly(A)) is designed for quick production of EZ Cap Reagent AG (3' OMe) capped, 5-moUTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of capping reagent (EZ Cap Reagent AG (3' OMe), Catalog No. B8178) using T7 RNA Polymerase. The capping of EZ Cap Reagent AG (3' OMe) results in a Cap 1 structure and ensures high translation efficacy. The addition of 5-moUTP (Catalog No. B8061) can suppress RNA-mediated innate immune activation. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.The kit contains sufficient reagents to carry out 25 reactions, 20 µL each time. Up to 25-180 µg of RNA can be generated with 1 µg of control template per standard reaction.
Additional Text
Short Description
Used for the in vitro synthesis of mRNA (EZ Cap Reagent AG (3' OMe) capped, 5-moUTP modified, poly(A) tailed)
Storage Note
Store all kit components at -20°C.
K1068 Protocol
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