Catalogue Number: KET6032-ABK
Manufacturer: | Abbkine |
Type: | Human ELISA Kit |
Alias: | TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7 |
Shipping Condition: | Blue Ice |
Unit(s): | 48 T, 96 T |
Range: | |
Sensitivity: | |
Sample type: | Cell Culture Supernatant, Other Biological Fluid, Plasma, Serum |
Sample size: | |
Application: | ELISA, sELISA |
Description: TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
TNF
7124
P01375
Colorimetric
EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate Human TNF-α.