Universal Loading Control Antibody Cocktail

Catalogue Number: KTD101-EN-ABK

Manufacturer:Abbkine
Type:Primary Antibody
Alias:Universal Loading Control Antibody Cocktail
Shipping Condition:Blue Ice
Unit(s): 1 kit
Application: WB

Description

Description: The Universal Internal Reference Antibody Cocktail is a set containing the antibodies β-actin, GAPDH and β-tubulin commonly used in immunoassay (WB, IHC-P, IF) as well as the antibody diluent. There are many kinds of internal reference antibodies. Therefore, the following three principles should be followed in choosing an appropriate internal reference antibody: 1.Considering the sample species, for mammalian tissues and cells, the commonly used reference proteins are β-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase, etc.For Plant samples, Plant Actin and RuBisCo are commonly used.Consider the molecular weight of the target protein. 2. The selected reference protein should have a different molecular weight from the target protein (>5 kDa) to distinguish the target protein bands. For high molecular weight proteins (over 100 kDa), we recommend that our clients use Vinculin as an internal reference; 2. Considering the expression and localization of target protein, select the corresponding internal reference, such as whole cell, nucleus, mitochondria, cell membrane and so on.

Additional Text

Short Description

Loading Control

Note

• Do not mix components from different Lot and manufacturers; Otherwise, the result may be abnormal. • To obtain the best experimental results, you need to optimize all of your experimental elements, including sample number, antibody concentration, membrane and sealant use. • Please select appropriate and stable reference antibodies according to the sample and target protein.As in adipose tissue, beta-tubulin may be unstable. • Please follow the detailed instructions of each component.