ExKine™ Pro Total Protein Extraction Kit for Plant Tissues
Catalogue Number: KTP3008-ABK
KTP3008 Image
KTP3008 Image
KTP3008 Image
KTP3008 Image
KTP3008 Image
Fig 1. Total proteins were extracted from different plants (Oryza, Arabidopsis). Lane left, proteins extracted by Abbkine Assay Kits. Lane right, proteins extracted by B-brand Assay Kits. After blocking, incubate with primary antibody (A01050, 1:5000), incubate secondary antibody (A21010, 1:10000), Chemiluminescence (BMU101-EN).
KTP3008 Image
Fig 2. Total proteins were extracted from different plants (Arabidopsis, Soybeans). Lane left, proteins extracted by Abbkine Assay Kits. Lane right, proteins extracted by B-brand Assay Kits. After blocking, incubate with primary antibody (A01070, 1:5000), incubate secondary antibody (A21010, 1:10000), Chemiluminescence (BMU101-EN).
KTP3008 Image
Fig 3. Total proteins were extracted from different plants (Oryza, Arabidopsis). Lane left, proteins extracted by Abbkine Assay Kits. Lane right, proteins extracted by B-brand Assay Kits. After blocking, incubate with primary antibody (A01110, 1:5000), incubate secondary antibody (A21010, 1:10000), Chemiluminescence (BMU101-EN).
KTP3008 Image
Fig 4. Plant protein profiles extracted by denaturing cell lysis buffer (Abbkine KTP3008). Extracted proteins were separated in 12% SDS-PAGE and stained with coomassie blue (BMU105-EN). Lane 1, soybean; lane 2, maize; lane 3, spinach; lane 4, Arabidopsis.
| Manufacturer: | Abbkine |
| Shelf Life: | 12 months |
| Type: | Protein Extraction Kit |
| Alias: | centrifugal column|Centrifuge tube|Column method|ExKine™ Pro Total Protein Extraction Kit for Plant Tissues|Extraction Kit|Protein Extraction|protein extrate|Total Protein|Total Protein Extraction |
| Shipping Condition: | RT |
| Storage Condition: | RT |
| Unit(s): | 5 T, 50 T |
| Range: | |
| Sensitivity: | |
| Sample type: | |
| Sample size: | |
| Application: | SDS-PAGE, WB |
Description
Description: ExKine™ Pro Plant Tissue Total Protein Extraction Kit (column method) is a new generation of ultra-fast protein extraction tool. More and more evidences show that the most commonly used RIPA buffer may lead to random loss of protein, resulting in many difficult data that are difficult to explain. ExKine™ Pro Total Protein Extraction Kit uses centrifugal column extraction technology combined with optimized lysis buffer to extract total protein more quickly and effectively, without grinding in liquid nitrogen, making WB results more accurate.
Additional Text
Short Description
ExKine™ Pro Total Protein Extraction Kit for Plant Tissues is a new generation of ultra-fast protein extraction tool that can efficiently extract total protein from plant tissues.
Note
1. Protease inhibitors are not necessarily added, but if downstream experiments take longer or are preserved for longer periods after protein extraction, it is advisable to add protease inhibitors. Protein concentration was determined by BCA method, but not by Bradford method. To study protein phosphorylation, phosphatase inhibitors should be added to the lysis buffer before use. 2. Before WB, still mix the sample and loading buffer to boiling. 3. The Plastic Rod is reusable, rinse thoroughly with deionized water, and dry it with paper towel. 4. If the protein concentration is low, increase the weight of tissues or reduce the volume of Denaturing Cell Lysis Buffer. 5. Partial tissues that is not completely lysed will not affect the quality of the sample.
KTP3008 Datasheet
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