PathPlus™ PRKCZ / PKC-Zeta Antibody (N-Terminus)
Catalogue Number: LS-A8392-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Shelf Life: | 12 months |
| Preservative: | 0.1% sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Synthetic 19 amino acid peptide near N-Terminus of human PRKCZ / PKC-Zeta. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (95%); Marmoset, Mouse, Rat, Rabbit (89%). |
| Application: | ELISA, IHC-P, IHC |
Additional Text
Concentration
1 mg/ml
Gene ID
5590
Gene Name
PRKCZ
Antigen Type
Synthetic Peptide
Purification
Affinity Purified
Protein: Target Region
N-terminus
Short Description
PKC-Zeta antibody LS-A8392 is an unconjugated rabbit polyclonal antibody to human PKC-Zeta (PRKCZ) (N-Terminus). Validated for ELISA and IHC.
Antibody Clonality
Polyclonal
Storage Note
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5-3 ug/ml.
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