PathPlus™ ERBB2 / HER2 Antibody (aa1243-1255)
Catalogue Number: LS-B2133-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.09% sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1 |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%); Elephant, Bat, Bovine (92%); Opossum (85%). |
| Application: | IHC-P, IP, WB, IHC-Fr, IHC |
Additional Text
Concentration
1 mg/ml
Gene ID
2064
Gene Name
ERBB2
Purification
Protein A purified
Short Description
HER2 antibody LS-B2133 is an unconjugated rabbit polyclonal antibody to HER2 (ERBB2) (aa1243-1255) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
Immunohistochemistry: LS-B2133 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2133 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 ug immunoprecipitated c-erbB-2 from 500 ug of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
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