IHC-plus™ EGFR Antibody (Internal)
Catalogue Number: LS-B2199-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | No preservative |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1 |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Sheep |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, IHC |
Additional Text
Gene ID
1956
Gene Name
EGFR
Antigen Type
Recombinant Protein
Concentration
2.2 mg/ml
Purification
Protein G purified
Protein: Target Region
Internal
Short Description
EGFR antibody LS-B2199 is an unconjugated sheep polyclonal antibody to EGFR (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Polyclonal
Application Notes
Immunohistochemistry: LS-B2199 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2199 was determined to be 5 ug/ml.
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