IHC-plus™ c-Met Antibody (Cytoplasmic Domain)
Catalogue Number: LS-B2812-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.09% sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide derived from cytoplasmic domain of human c-Met protein |
| Application: | IHC-P, IHC-Fr, IHC |
Additional Text
Gene ID
4233
Gene Name
MET
Antigen Type
Synthetic Peptide
Concentration
1.14 mg/ml
Purification
Purified
Storage Note
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Antibody Clonality
Monoclonal
Short Description
C-Met antibody LS-B2812 is an unconjugated mouse monoclonal antibody to human c-Met (Cytoplasmic Domain). Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application Notes
Immunohistochemistry: LS-B2812 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2812 was determined to be 10 ug/ml. Positive control: Breast cancer.
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