IHC-plus™ EGFR Antibody (Internal)

Catalogue Number: LS-B2914-LSP

Manufacturer:	LifeSpan BioSciences Inc.
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Shipping Condition:	RT
Unit(s):	50 ug
Host name:	Sheep
Clone:
Isotype:
Immunogen:	A synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region
Application:	ICC, IHC-P, IP, WB, IHC

Overview

Additional Text

Concentration

1 mg/ml

Gene ID

1956

Gene Name

EGFR

Antigen Type

Synthetic Peptide

Purification

Protein G purified

Protein: Target Region

Internal

Short Description

EGFR antibody LS-B2914 is an unconjugated sheep polyclonal antibody to EGFR (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Storage Note

Store at -20°C. Avoid freeze-thaw cycles.

Antibody Clonality

Polyclonal

Application Notes

Immunohistochemistry: LS-B2914 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2914 was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration.

Price

£572.00

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