IHC-plus™ PECAM-1 / CD31 Antibody (clone 2F7B2)
Catalogue Number: LS-B3446-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.03% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | 2F7B2 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human CD31 expressed in E. Coli. |
| Application: | ELISA, IF, IHC-P, WB, IHC |
Additional Text
Gene Name
PECAM1
Concentration
1 mg/ml
Gene ID
5175
Antigen Type
Recombinant Protein
Antibody Clonality
Monoclonal
Storage Note
Short term: store at 4°C. Long term: store at -20°C.
Application Notes
Immunohistochemistry: LS-B3446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3446 was determined to be 1:200.
Short Description
CD31 antibody LS-B3446 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
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