IHC-plus™ MLH1 Antibody (clone 4C9C7)
Catalogue Number: LS-B3475-LSP
| Manufacturer: | LifeSpan BioSciences Inc. |
| Preservative: | 0.03% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | MLH1, COCA2, FCC2, HMLH1, HNPCC, HNPCC2, MutL protein homolog 1 |
| Shipping Condition: | RT |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | 4C9C7 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
| Application: | ELISA, IF, IHC-P, WB, IHC |
Additional Text
Gene ID
4292
Gene Name
MLH1
Short Description
MLH1 antibody LS-B3475 is an unconjugated mouse monoclonal antibody to human MLH1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Antibody Clonality
Monoclonal
Storage Note
Short term: store at 4°C. Long term: store at -20°C.
Application Notes
Immunohistochemistry: LS-B3475 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3475 was determined to be 1:200.
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